منابع مشابه
Resolution scaling in STED microscopy.
We undertake a comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal depletion patterns. We used an experimental platform featuring a high quality depletion pattern which results in operation close to the optimal optical performance. Its superior i...
متن کاملSub-Abbe resolution: from STED microscopy to STED lithography
Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission...
متن کاملSTED microscopy: increased resolution for medical research?
Optical imaging is crucial for addressing fundamental problems in all areas of life science. With the use of confocal and two-photon fluorescence microscopy, complex dynamic structures and functions in a plethora of tissue and cell types have been visualized. However, the resolution of 'classical' optical imaging methods is poor due to the diffraction limit and does not allow resolution of the ...
متن کاملTime-gating improves the spatial resolution of STED microscopy.
Stimulated-emission depletion (STED) microscopy improves image resolution by encoding additional spatial information in a second stimulated-decay channel with a spatially-varying strength. Here we demonstrate that spatial information is also encoded in the fluorophore lifetime and that this information can be used to improve the spatial resolution of STED microscopy. By solving a kinetic model ...
متن کاملSTED microscopy reveals crystal colour centres with nanometric resolution
Because they have spin states that can be optically polarized and detected, fluorescent nitrogen vacancies in diamond1–3 have considerable potential for applications in quantum cryptography4,5 and computation6–8, as well as for nanoscale magnetic imaging9,10 and biolabelling11,12. However, their optical detection and control are hampered by the diffraction resolution barrier of far-field optics...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2008
ISSN: 1094-4087
DOI: 10.1364/oe.16.004154